Effect of Qurs-e-Rewand (A Hepatoprotective Unani Formulation) on Pentobarbitone induced Sleeping in Mice

Background: The present study was planned to investigate the effect of Qurse Rewand commonly used compound hepatoprotective drug on sleep duration in sodium pentobarbital (PB)-induced sleeping in mice. Methodology: The animals were divided into five groups of 6 animals each and they were fasted overnight. The animals of group I and II were left untreated, whereas the animals kept in group III, IV and V were administered, Silymarin (100 mg/kg b. w.) Extract of Qurse Rewand (50mg/kg b. w.) and (100 mg/kg b. w.) respectively as single and double dose orally for 7 days. On the 8 th day the animals in group II, III, IV and V were given CCl4 in dose of 2 ml/kg b. w. intraperitonial route. Two hours later the animals in all the five groups were administered 30 mg/ kg b. w. of sodium pentobarbitone I.P. The sleeping time was recorded by observing the CNS activity as pinna, sound and righting reflex in each animal at every five minutes. Results: The findings obtained, indicated the significant reduction (P ≤ 0.05-0.001) in the onset and duration of pentobarbitone-induced sleep in mice treated with the test drug of both the doses and it was found significant against CCl4+Pentobarbitone induced animals. The results suggest that Qurse Rewand shortened the pentobarbital hypnosis without major toxic effect and the result exhibited by the double dose of QR was more potent than single dose and shortening of duration is almost equal to the standard drug. Conclusion: On the basis of reduction in sleeping time it can be concluded that the test drug is quite safe and has wide therapeutic index and therefore can be safely used in high doses also.